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Allen Institute Research Associate II/III - Flow Cytometry in Seattle, Washington

The new Allen Institute for Immunology is looking for a Research Associate II/III (RA II/III, based on experience). The goal of the Allen Institute for Immunology is to advance the fundamental understanding of human immunology through the study of immune health and disease where excessive or impaired immune responses drive pathological processes. The RAII/III will be part of a multi-disciplinary team and will be responsible for performing core immunology and basic lab techniques for the Allen Institute in a standardized, high throughput manner. As an early member of the new team, this RA II/III will support delivery of archival quality flow cytometry data sets, assist with instrument performance monitoring and high parameter panel building, as well as participate in the general operation and maintenance of the laboratory. Opportunities exist for the exceptional candidate to actively contribute to the analysis and interpretation of data from our longitudinal healthy, autoimmune, and cancer cohorts as well as cross train in molecular biology techniques such as single cell sequencing. The RA II/III is an advanced position responsible for executing a wide variety of laboratory tasks and/or experiments in a high quality production environment following Standard Operating Procedures (SOPs). Good judgment and problem solving skills are required for recognizing process deviations, participating in troubleshooting, and operation of complex laboratory instrumentation. The RA II/III is expected to comply with regulatory requirements, adhere to numerous SOPs, be capable of successfully contributing in a near GLP environment, and to work efficiently in a team environment. A successful candidate possesses excellent attention to detail and the eagerness to work in a Team Science, deadline-driven atmosphere. JOB RESPONSIBILITIES Perform Flow Cytometry (cell counting and sorting, detection of biomarkers and cell phenotyping), Support testiing, optimization, and troubleshooting of new flow cytometry reagents. Perform Quality Control assays where necessary. Help with assay validation, SOP authoring and optimizations. Validate equipment and tracking tools used. Meet experimental and data production schedules. Keep excellent experimental records of experiments Follow standard operation procedures and good laboratory practices. Accurately record process notes. Support discovery projects as a part of the Experimental Immunology core team. Help set up and operate liquid handling instruments and other automation Other duties as assigned. REQUIRED QUALIFICATIONS Bachelor's degree in a life science Experience with analysis and sorting on a flow cytometer. Working knowledge of flow cytometry automation. Direct experience with flow cytometry analysis, using FlowJo or equivalent. Level II: 2 to 5 years of relevant post-undergraduate research experience Level III: 5+ years of relevant post-undergraduate research experience PREFERRED QUALIFICATIONS Background in immunology; working knowledge of cell types and functional assays Experience with cell culture techniques, especially relating to T, B, and/or APC assays Strong organizational and communication skills Attention to detail and strong problem solving skills. Experience with a LIMS system Excellent work ethic displayed as a reliable, self-motivated, enthusiastic team player. 1+ year experience working in a GLP or CLIA regulated environment Ability to learn new software programs. Experience with FACS Eager to learn new skills. **Please note, this opportunity does not sponsor work visas and

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