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Children's Hospital Boston Research Assistant II in Boston, Massachusetts

52738BRTitle:Research Assistant IIDepartment:Medicine-EndocrinologyAutoReqId:52738BRStatus:Full-TimeStandard Hours per Week:40 Job Posting Description:At Boston Children’s Hospital, success is measured in patients treated, parents comforted and teams taught. It’s in discoveries made, processes perfected, and technology advanced. In major medical breakthroughs and small acts of kindness. And in colleagues who have your back and patients who have your heart. As a teaching hospital of Harvard Medical School, our reach is global and our impact is profound. Join our acclaimed Division of Endocrinology and discover how your talents can change lives. Yours included.

The Breault Laboratory at Boston Children’s Hospital is focused on identifying mechanisms underlying tissue and stem cell homeostasis in self-renewing tissues, such as intestine, adrenal gland, and bone using mouse, human and cell culture models. We are seeking an enthusiastic and experienced full-time Research Associate position in the Gastrointestinal Organoid Derivation and Culture Core Facility of the Harvard Digestive Disease Center at Boston Children’s Hospital. Past research assistants in the lab have gone on to prestigious graduate and medical schools as well as positions within the biomedical/pharma industry. Boston Children’s Hospital cannot offer visa sponsorship for this opportunity.

This Research Assistant II will be responsible for:

  • Establishing and culturing of human/mouse intestinal organoids.

  • Conditioned media preparation.

  • Immunohistochemistry.

  • FACS analysis.

  • Supply ordering.

  • Documenting, compiling and analyzing experimental data with meticulous record keeping.

  • Training others in organoid culture techniques and collaborating with outside laboratories.

In order to qualify, you must have:

  • BS/BA and at least 1 year of related laboratory experience.

  • Prior cell culture experience is essential and experience in isolating primary cells and/or organoid culture is desirable.

  • Willingness to commit to at least 2 years to this position.

  • In addition, competence in the following techniques is highly preferred: immunohistochemistry, fluorescence microscopy, animal dissection, CRISPR/Cas9, and use of viral vectors in cell culture.

  • Must be able to work independently as well as in a team environment.

  • Ability to troubleshoot, self-motivate, strong organizational and communication skills, and a willingness to accept challenges are highly desirable.

Boston Children’s Hospital offers competitive compensation and unmatched benefits, including a rotating days/evenings and week-end schedule, affordable health, vision and dental insurance, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition Reimbursement, cell phone plan discounts and discounted rates on T-passes (50% off).

Boston Children’s Hospital is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability.Office/Site Location:Boston