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Joslin Diabetes Center Assistant Manager, Research (Molecular Phenotyping Core) in Boston, Massachusetts

The primary responsibility of the Assistant Manager will be to assist the Joslin research division with implementation and processing of samples provided by Joslin investigators for transcriptomic and metabolic analysis within the framework of the Molecular Phenotyping Core Laboratory.

The major focus will be to implement single-cell sequencing for the Joslin and external research communities. This will include quality analysis of single-cell suspensions, generating single-cell GEMS, performing RT, generating libraries for RNA-sequencing, and working with the bioinformatics core to perform quality control analyses.

The Assistant Manager will also provide oversight of PCR equipment (both RT-PCR and digital PCR), single-cell sequencing platform, Seahorse bioanalyzers, Tissue Lyser, microscope, and spectrophotometers, including ensuring maintenance of equipment, quality control, documentation, and oversight of billing to users.

In addition, the incumbent will interact with investigators and fellows, working with them on sample preparation and analysis, use of the digital and real-time PCR instruments, and use of the Seahorse bioanalyzer platform. The research assistant will organize training sessions and will be asked to present at seminars and lab meetings.

  • Assisting with maintaining the equipment in the Molecular Phenotyping Core Laboratory.

  • Provide oversight of equipment, budget, and sign-up procedures.

  • Assist with maintaining the core database and analysis software.

  • Perform data transfer, backup, safeguarding operations.

  • Prepare buffers and reagents; help maintain stocks of consumables for the facility, and place orders to replenish stocks as necessary.

  • Establish new procedures and policies and modify existing ones as necessary to maintain up-to-date best practices and optimize core operation.

  • Accurately review and approve billing for internal core users, and generate invoices for external users, on a monthly basis in conjunction with the Office of Sponsored Research.

  • Process investigator-provided cell suspensions for single-cell analysis. Perform quality control analysis at each step.

  • Assist scientists with experimental design and analysis (real-time qRT-PCR, digital PCR, single cell analysis, bioenergetics analysis, library preparation).

  • Guide investigators with the best choice of reagents, protocols, etc with regards to instrumentation.

  • Provide assistance and feedback to investigators with data analysis.

  • Troubleshoot problems with software and instrumentation, and assist investigators with troubleshooting of their experiments and data.

  • Collect and present core usage data to the core director

  • Attend internal research seminars to keep abreast of current research at Joslin.

  • Order supplies as needed, while maintaining chargeback account records and interface with Finance/Information Technology regarding operation of online ordering system.

  • Work extended hours, weekends and holidays on an exceptional basis, as agreed upon with the Core Director.

  • Enforce adherence to core policies and procedures among users and core operators.

  • Attend quarterly core budget meetings at the request of the Core Director and advise on budget-related decisions based on instrument usage, maintenance needs, and opportunities for improvement or enhancement of core capabilities.

  • Assist with writing and preparing applications for core-related grants.

  • Collect and present core usage data to the core director.

  • Other duties as assigned.

  • Bachelor’s degree in biology, molecular biology, or related field. Master’s degree preferred.

  • Minimum of 2 years’ experience relevant to essential responsibilities of the job.

  • Previous practical Molecular Biology experience preferred: including RNA processing, epigenetics, RNA sequencing, and library preparation.

  • Proficient computer skills, including Microsoft Office Suite and familiarity with omics database management preferred

  • Adhere to lab safety protocols when working with biological and other hazardous materials.

  • Strong interpersonal and communication skills are a must.

Joslin Diabetes Center is an Equal Opportunity Employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, genetic information, disability status, protected veteran status or any other status protected by law.

Requisition Number (add R/N and FY after populates): R3222-19

External Company URL:

Street: One Joslin Place